INFLUENCE OF MELATONIN ON AGE-RELATED CHANGES IN CARBOHYDRATE METABOLISM AND ANTIOXIDANT CAPACITY IN THE BLOOD OF ALLOXAN DIABETIC RATS

Melatonin and its metabolites have potent antioxidant/anti-inflammatory properties, they proven to be highly effective in a variety of disorders linked inflammation oxidative stress. The object this experimental research was ascertain the influence aging on level basal glycemia activities glucose-6-phosphate dehydrogenase [EC1.1.1.49], pyruvate kinase [EC 2.7.1.40] glutathione reductase [EC1.6.4.2] erythrocytes alloxan diabetic rats background melatonin injections. Methods: We used 100 male Wistar rats, two age groups: - 2-month (adult), II 4-month (old). Alloxan diabetes evoked via injecting with 5% solution monohydrate intraperitoneally dose 170 mg/kg. Four days after induction, were divided into (untreated) melatonin-diabetic group (10 mg/kg, daily for six weeks). Blood taken from tail vein evaluate 5-th 47-th day injection alloxan. Rats sacrificed at experiment accordance ethical treatment animals. Determinations enzymes by standard methods. Statistical analysis performed using Statistica 10 StatSoft Inc. Results. fifth animals both groups increased average 115% baseline values. founded that index higher old 20% more than adult rats. Pyruvate activity decreased 34% 51% respectively compared control. 25% 44% control day. changes may result age-related glucose metabolism due disturbances free radical mechanisms. Glutathione 30% 36% A 42-days contributed normalization glycemia, rat blood, as well considerable increase dehydrogenase, whose exceeded 9% particular Under blood increasing number substrate (stimulating flow cells phosphorylation) direct action. Conclusion. In case probably increases use regeneration NADPH2 aerobic oxidation indicate an acceleration antioxidative protection energy production